[논문/International Journal of Molecular Sciences] Effects of Loganin on Bone Formation and Resorption In Vitro and In Vivo
작성자
관리자작성일자
2022-11-18 08:25조회수
53KIURI 이창근 참여연구원 논문
International Journal of Molecular Sciences. 2022 Nov 16; doi: 10.3390/ijms232214128
Effects of Loganin on Bone Formation and Resorption In Vitro and In Vivo
Osteoporosis is a disease caused by impaired bone remodeling that is especially prevalent in elderly and postmenopausal women. Although numerous chemical agents have been developed to prevent osteoporosis, arguments remain regarding their side effects. Here, we demonstrated the effects of loganin, a single bioactive compound isolated from Cornus officinalis, on osteoblast and osteoclast differentiation in vitro and on ovariectomy (OVX)-induced osteoporosis in mice in vivo. Loganin treatment increased the differentiation of mouse preosteoblast cells into osteoblasts and suppressed osteoclast differentiation in primary monocytes by regulating the mRNA expression levels of differentiation markers. Similar results were obtained in an osteoblast–osteoclast co-culture system, which showed that loganin enhanced alkaline phosphatase (ALP) activity and reduced TRAP activity. In in vivo experiments, the oral administration of loganin prevented the OVX-induced loss of bone mineral density (BMD) and microstructure in mice and improved bone parameters. In addition, loganin significantly increased the serum OPG/RANKL ratio and promoted osteogenic activity during bone remodeling. Our findings suggest that loganin could be used as an alternative treatment to protect against osteoporosis.
Keywords: loganin; bioactive compound; anti-osteoporosis agent; bone remodeling
