KIURI 이창근 참여연구원 논문
Biomedicine & Pharmacotherapy. 2024 May. doi: doi.org/10.1016/j.biopha.2024.116799
Anti-obesity effects of Celosia cristata flower extract in vitro and in vivo
Highlights
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CC inhibits lipid droplet accumulation and adipocyte differentiation.
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The treatment with CC exhibits a protective effect on hepatic steatosis, adipocyte hypertrophy, and elevated liver enzymes.
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CC extract improves glucose tolerance, insulin activity, and lipid profiles.
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This study provides potential benefits of CC in obesity management.
Abstract
Background
The overstoring of surplus calories in mature adipocytes causes obesity and abnormal metabolic activity. The anti-obesity effect of a Celosia cristata (CC) total flower extract was assessed in vitro, using 3T3-L1 pre-adipocytes and mouse adipose-derived stem cells (ADSCs), and in vivo, using high-fat diet (HFD)-treated C57BL/6 male mice.
Methods
CC extract was co-incubated during adipogenesis in both 3T3-L1 cells and ADSCs. After differentiation, lipid droplets were assessed by oil red O staining, adipogenesis and lipolytic factors were evaluated, and intracellular triglyceride and glycerol concentrations were analyzed. For in vivo experiments, histomorphological analysis, mRNA expression levels of adipogenic and lipolytic factors in adipose tissue, blood plasma analysis, metabolic profiles were investigated.
Results
CC treatment significantly prevented adipocyte differentiation and lipid droplet accumulation, reducing adipogenesis-related factors and increasing lipolysis-related factors. Consequently, the intracellular triacylglycerol content was diminished, whereas the glycerol concentration in the cell supernatant increased. Mice fed an HFD supplemented with the CC extract exhibited decreased HFD-induced weight gain with metabolic abnormalities such as intrahepatic lipid accumulation and adipocyte hypertrophy. Improved glucose utilization and insulin sensitivity were observed, accompanied by the amelioration of metabolic disturbances, including alterations in liver enzymes and lipid profiles, in CC-treated mice. Moreover, the CC extract helped restore the disrupted energy metabolism induced by the HFD, based on a metabolic animal monitoring system.
Conclusion
This study suggests that CC total flower extract is a potential natural herbal supplement for the prevention and management of obesity.
